Figure 2
Purification and in-gel chitinase activity assay of OsCLP. A, Coomassie blue-stained 12% SDS-PAGE of purified OsCLP. The eluted fractions from the Ni2+-affinity resins contained highly purified, soluble OsCLP. A plus sign indicates that IPTG was included in the growth medium. B, chitinase activity assay. Protein samples were separated on 12.5% SDS-PAGE gels containing 1% glycol chitin substrate and stained with 0.01% Fluorescent Brightener 28 staining solution.