Table 4 Steady-state kinetic parameters of ChU-B mutant expressed in P. pastoris and S. cerevisiae
From: Functional expression of a blood tolerant laccase in Pichia pastoris
Substrate | pH | ChU-B fromP. pastoris | ChU-B fromS. cerevisiae | (Parental type, OB-1mutanta) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
Km(mM) | kcat(s-1) | kcat/Km(mM-1s-1) | Km(mM) | kcat(s-1) | kcat/Km(mM-1s-1) | Km(mM) | kcat(s-1) | kcat/Km(mM-1s-1) | ||
ABTS | 3.0 | 0.024 ± 0.002 | 22.3 ± 0.5 | 929 | 0.023 ± 0.001 | 57.0 ± 0.9 | 2478 | 0.0063 ± 0.0009 | 200 ± 7 | 31746 |
7.4 | 0.26 ± 0.01 | 1.13 ± 0.02 | 4.35 | 0.32 ± 0.02 | 3.09 ± 0.07 | 9.66 | n.d. | n.d. | n.d. | |
DMP | 5.0 | 1.91 ± 0.05 | 5.65 ± 0.05 | 2.96 | 2.0 ± 0.2 | 19.5 ± 0.5 | 9.75 | 0.14 ± 0.02 | 134 ± 5 | 957 |
7.4 | 0.23 ± 0.03 | 0.41 ± 0.02 | 1.78 | 0.22 ± 0.03 | 1.95 ± 0.05 | 8.86 | n.d. | n.d. | n.d. | |