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Table 4 Steady-state kinetic parameters of ChU-B mutant expressed in P. pastoris and S. cerevisiae

From: Functional expression of a blood tolerant laccase in Pichia pastoris

Substrate pH ChU-B fromP. pastoris ChU-B fromS. cerevisiae (Parental type, OB-1mutanta)
Km(mM) kcat(s-1) kcat/Km(mM-1s-1) Km(mM) kcat(s-1) kcat/Km(mM-1s-1) Km(mM) kcat(s-1) kcat/Km(mM-1s-1)
ABTS 3.0 0.024 ± 0.002 22.3 ± 0.5 929 0.023 ± 0.001 57.0 ± 0.9 2478 0.0063 ± 0.0009 200 ± 7 31746
7.4 0.26 ± 0.01 1.13 ± 0.02 4.35 0.32 ± 0.02 3.09 ± 0.07 9.66 n.d. n.d. n.d.
DMP 5.0 1.91 ± 0.05 5.65 ± 0.05 2.96 2.0 ± 0.2 19.5 ± 0.5 9.75 0.14 ± 0.02 134 ± 5 957
7.4 0.23 ± 0.03 0.41 ± 0.02 1.78 0.22 ± 0.03 1.95 ± 0.05 8.86 n.d. n.d. n.d.
  1. a Data from Maté et al., 2010. n.d. not determined.