Figure 5

Inhibition of ChU-B by halides. (A) NaF inhibition with (i) ABTS at pH 4.0 (X-axis in μM; black circles, ChU-B from P. pastoris; white circles, ChU-B from S. cerevisiae); (ii) DMP at pH 5.0 (X-axis in mM; black squares, ChU-B from P. pastoris; white squares, ChU-B from S. cerevisiae; (iii) ABTS at pH 7.4 (X-axis in mM; black diamonds, ChU-B from P. pastoris; white diamonds, ChU-B from S. cerevisiae). (B) NaCl inhibition with (i) ABTS at pH 4.0 (black circles, ChU-B from P. pastoris; white circles, ChU-B from S. cerevisiae); (ii) DMP at pH 5.0 (black squares, ChU-B from P. pastoris; white squares, ChU-B from S. cerevisiae). (C) NaCl inhibition at physiological pH (7.4) with ABTS as substrate (black bars, ChU-B from P. pastoris; grey bars, ChU-B from S. cerevisiae).