Figure 4
From: Less is more: strategies to remove marker genes from transgenic plants
PCR screening of Cre- lox -mediated SMG excision events in plant genome. (A) Using primers P1 and P2 designed from outside the two loxP sites, PCR product ‘X’ (parental band) was amplified when no excision occur, while ‘Y’ band is produced when excision events occur. (B) Lane 1: DNA markers, lane 2: parental band (X), lane 3: positive control band (Y) (obtained from transient assay in bacterial cells), lanes 4–9: excision event detection, lane 10: no DNA. Abbreviations. RB: T-DNA right border, LB: T-DNA left border, GOI: gene of interest, SMG: selectable marker gene.