Figure 3

Strategy for using Ac transposon element to produce SMG-free transgenic plants. (A) T-DNA contains both the GOI (flanked by transposase-recognizing sites) and the transposase is transformed into plants. When the transposase is activated, the GOI cassette will be excised and insert into a different locus. If the GOI cassette lands at an unlinked locus, the SMG-free transgenic plants can be obtained after segregation in the subsequent generation. (B) T-DNA containing both the SMG (flanked by transposase-recognizing inverted repeat sequences) and the GOI is transformed into plants. When transposase activity is provided, the SMG cassette can move away and insert into a different locus. If the SMG and GOI cassettes land at an unlinked locus, the SMG-free transgenic plants can be obtained after genetic segregation in the sequential generation. Abbreviations. RB: T-DNA right border, LB: T-DNA left border, GOI: gene of interest, SMG: selectable marker gene. P₁, P₂ and P₃: promoters, T₁, T₂ and T₃: terminators.