Figure 2
From: Less is more: strategies to remove marker genes from transgenic plants
Positive-negative selection scheme in producing SMG-free transgenic plants. A negative selectable marker gene (‘N’) is build next to a positive selectable marker gene (SMG), SMG-N, as depicted in the figure. After genetic transformation, explants are first selected with the positive selectable marker. Seeds of next generation are then selected with a negative selectable marker to remove 50% plants which still have SMG-N. PCR is then followed to screen marker-free transgenic plants. Abbreviations. RB: T-DNA right border, LB: T-DNA left border, GOI: gene of interest.