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Table 1 Primers for PCR amplification

From: Influence of untranslated regions on retroviral mRNA transfer and expression

Target Primer Primer Sequence (5′-3′) Sequence Length
Luciferase Luciferase-S 5′-TCgAAgCTTACCATggAAgACgCCA -3′ 1677 bp
Luciferase-AS 5′-gAATggTACCTTACAATTTggAC -3′
Vimentin Vimentin-5UTR-AS 5′ ATCggCTAgCgCgTCCCCgCgCCAg -3′ 144 bp
Vimentin-5UTR-AS 5′-gAATAAgCTTggCTgCggAgggT -3′
Vimentin-3UTR-S 5′-ATCgggTACCAAATTgCACACAC -3′ 325 bp
Vimentin-3UTR-AS 5′-gAATgCggCCgCgAAgCAgAACC -3′
Myosin Myosin-5UTR-S 5′-TCggCTAgCgAAggCTAAgCA -3′ 154 bp
Myosin-5UTR-AS 5′-AATTAAgCTTACCTgAACCTg -3′
Myosin-3UTR-S 5′-ATCgggTACCgCCTCTTCTCCTg -3′ 1389 bp
Myosin-3UTR-AS 5′-gAATgCggCCgCgTgATgCTCAg -3′
MLV Packaging signal psi-S 5′ = AAAgTggTACCgggAggTAAgCT-3′ 322 bp
psi-AS 5′-gCCTTggTACCgAACTgTTTTAg-3′
  1. Sequence amplified for modifying the vectors (restriction sites used allowing the cloning are in bold).