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Table 1 Primers for PCR amplification

From: Influence of untranslated regions on retroviral mRNA transfer and expression

Target

Primer

Primer Sequence (5′-3′)

Sequence Length

Luciferase

Luciferase-S

5′-TCgAAgCTTACCATggAAgACgCCA -3′

1677 bp

Luciferase-AS

5′-gAATggTACCTTACAATTTggAC -3′

Vimentin

Vimentin-5UTR-AS

5′ ATCggCTAgCgCgTCCCCgCgCCAg -3′

144 bp

Vimentin-5UTR-AS

5′-gAATAAgCTTggCTgCggAgggT -3′

Vimentin-3UTR-S

5′-ATCgggTACCAAATTgCACACAC -3′

325 bp

Vimentin-3UTR-AS

5′-gAATgCggCCgCgAAgCAgAACC -3′

Myosin

Myosin-5UTR-S

5′-TCggCTAgCgAAggCTAAgCA -3′

154 bp

Myosin-5UTR-AS

5′-AATTAAgCTTACCTgAACCTg -3′

Myosin-3UTR-S

5′-ATCgggTACCgCCTCTTCTCCTg -3′

1389 bp

Myosin-3UTR-AS

5′-gAATgCggCCgCgTgATgCTCAg -3′

MLV Packaging signal

psi-S

5′ = AAAgTggTACCgggAggTAAgCT-3′

322 bp

psi-AS

5′-gCCTTggTACCgAACTgTTTTAg-3′

  1. Sequence amplified for modifying the vectors (restriction sites used allowing the cloning are in bold).