Luciferase expression from Non-viral (A) and Truncated-retroviral (B) constructs. 293FT cells were transfected with the different constructs. One day after transfection, transcription was blocked by actinomycin D. 2 to 4 days post-transfection, luciferase activity was measured in lysed cells. Number of experiments, N = 3. (3A) The analysis was performed using pcDNALuc-psi as comparator for each condition. To evaluate the significance of the observed differences, we performed a one sample t-test. This statistical test allowed us to determine if the means observed for pcDNA-Luc-Vimentin-psi and pcDNA-Luc-NMHC-psi were significantly different to the reference value fixed to 1, which correspond to pcDNA-Luc-psi. *p-value <0.05. (3B) As the signal from pCMV-5′LTR-psi-Luc was not different from the one from pCMV-5′LTR-psi-Luc-Vimentin, we evaluated the significance of the observed differences between pCMV-5′LTR-psi-Luc-Vimentin and pCMV-5′LTR-psi-Luc-NMHC, this was achieved with a Wilcoxon-Mann–Whitney. *p-value < 0.05.