Figure 4
From: Modular glycosphere assays for high-throughput functional characterization of influenza viruses
Assay performance characteristics. A, Assay reproducibility as determined by three independent receptor binding assays performed in different weeks with independently prepared reagents and non-identical virus stocks of A/Fort Monmouth/1/1947(H1N1). B, Limit of detection as determined by comparing the fluorescent signals of increasing viral titers of A/Hong Kong/8/1968(H3N2) with the no-virus control (NV). Statistical significance was determined by an unpaired t-test and categorized as not significant (ns), p < 0.05 (*), p < 0.01 (**), or p < 0.001 (***). C, Assay robustness as determined by comparing the signal intensity of identical viral titers in different assay media. A/Fort Monmouth/1/1947(H1N1) was well detected over a range of viral titers when serially diluted in phosphate buffered saline (PBS), virus growth medium (MEM), or Universal Transport Medium (UTM).