Figure 3
From: One single method to produce native and Tat-fused recombinant human α-synuclein in Escherichia coli
Gel permeation analysis of recombinant TAT-AS. Peak 1 corresponds to an apparent molecular mass of 130 ± 8 kDa and peak 2 corresponds to an apparent molecular mass of 61.4 ± 5.4 kDa; both peaks gave a single band at 18 kDa in SDS-PAGE analysis (inset). Conditions: HiLoad 16/60 Superdex 200 (GE Healthcare) column; 20 mM potassium phosphate buffer, pH 7.4, 150 mM sodium chloride as elution buffer.