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Figure 5 | BMC Biotechnology

Figure 5

From: Baculovirus-mediated gene transfer in butterfly wings in vivo: an efficient expression system with an anti-gp64 antibody

Figure 5

Baculovirus-mediated GFP expression in J. orithya pupae and adults obtained from baculovirus-injected and antibody-injected individuals. (A) An adult ventral forewing. The boxed region is enlarged in B. (B) High magnification of A. The boxed region is enlarged in C and D. (C) High magnification of B under blue light and a small degree of white light. Intense fluorescence was observed in regions where no scales were found, but some scales also fluoresced. (D) High magnification of C under blue light. (E) An adult dorsal hindwing, isolated from a pupal case due to eclosion failure. The circled region is enlarged in G and H. (F) The same wing in E under blue light. A few clusters of green fluorescence were observed, as indicated by circles. (G) High magnification of E. (H) The same region as G. The circled region is magnified in I. (I) High-magnification confocal image of H. Intensive fluorescence was observed in scales. (J) An adult ventral forewing, isolated from a pupal case due to eclosion failure. (K) The same wing as J under blue light. No green fluorescence was detected. (L) The same wing as J after the removal of scales. (M) The same wing as L, with scales removed, under blue light. Green fluorescence was observed throughout the wing. (N) A pupa with fluorescence in a section of the proboscis and abdomen (circled). The wing shown in E was obtained from this pupa. (O) An eclosed adult from the pupa in N. A section of the proboscis (boxed) and abdomen (circled) shows green fluorescence, as predicted at the pupal stage. (P) A pupa with green fluorescence in a part of a leg (boxed). (Q) An eclosed adult from the pupa in P. A part of a leg shows fluorescence, as predicted at the pupal stage.

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