Figure 1
Baculovirus-mediated GFP expression in J. orithya pupae. Baculovirus was injected at the pupal stage 6–12 hours after pupation. (A) A whole pupa under the bright field showing the GI level. The injection consisted of 2.0 μL at 2.5 × 102 pfu/mL. This individual is identical to C. (B-G) Whole pupae under blue light showing various GFP fluorescent levels. (B) G0. No injection (normal pupa) (C) GI. (D) GI. The injection consisted of 2.0 μL at 5.0 × 105 pfu/mL. (E) GII. The injection consisted of 2.0 μL at 2.0 × 105 pfu/mL. (F) GIII. The injection consisted of 2.0 μL at 5.0 × 105 pfu/mL. The level of GFP fluorescence varied even if the same conditions were used in D and F. (G) GIII. The injection consisted of 2.0 μL at 1.0 × 107 pfu/mL. (H) Higher magnification of the wing region of the fluorescing pupa shown in D.