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Table 1 Effects of metal ions and chemicals on the activity of the pectate lyase

From: The alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastorisis more stable and efficient for degumming ramie fiber

Metal ions or chemicals

Concentration

Relative activity(%)

No addition

0 mM

100.0

K+ (KCl)

5 mM

102.8 ± 2.325

Zn2+ (ZnSO4)

5 mM

34.5 ± 3.657

Fe3+ (FeCl3)

5 mM

58.7 ± 0.562

Mg2+ (MgCl2)

5 mM

35.5 ± 1.246

Mn2+ (MnCl2)

5 mM

8.5 ± 1.21

Co2+ (CoCl2)

5 mM

47.0 ± 4.341

Hg2+ (HgCl2)

5 mM

0

Cu2+ (CuSO4)

5 mM

6.6 ± 1.564

Ca2+ (CaCl2)

5 mM

138.3 ± 3.564

Ni2+(NiSO4)

5 mM

81.8 ± 0.957

Li+ (LiCl)

5 mM

103.3 ± 2.451

Sr2+(SrCl2)

5 mM

104.1 ± 1.367

EDTA

5 mM

0

SDS

0.5%

0

Tween-80

0.5%

100.7 ± 3.124

DMSO

0.5%

124.8 ± 4.658

methanol

0.5%

98.3 ± 2.324

ethanol

0.5%

103.4 ± 1.246

isopropyl

0.5%

99.6 ± 2.154

alcohol

0.5%

101.5 ± 1.245