Skip to main content

Table 3 PCR primers used in this study

From: Development of a Fur-dependent and tightly regulated expression system in Escherichia colifor toxic protein synthesis

Name

Sequence (5’-3’)a

PFhuA1-for

CGGAATTCCAGTACCAGTGCCGCCATCGTCCA

PFhuA1-rev

TGATTATCATTATCAGCAAAAAATTGCGAATCTTTCTC

GFP1-for

ATAATGATAATCATTATCACTAAAATTGCGTAAAATTAAT

GFP1-rev

AAAACTGCAGTTATTTGTACAGTTCATCCATGCCA

PFhuA2-for

CGGAATTCCCTTAATGAAAGTAATTGAGAAAGAT

GFP2-for

AACGATTCTCAAATCTATAGCAGAGGTTTTATCAT

GFP2-rev

AAAACTGCAGTTATTTGTACAGTTCATCCATGC

PFhuA3-for

CGGAATTCACCAGTACCAGTGCCGCCATCGTC

PFhuA3-rev

TAATGATTATCATTATCTCAATTACTTTCATTAAGGCACC

GFP3-for

ATGATAATCATTATCGCTTTTAATTAAGATAATTATCACT

GFP3-rev

AAAACTGCAGTTATTTGTACAGTTCATCCATGCCAT

Fur-for

CAGCTGGAGCAAATTCTGTCACTTCTTCTA

Fur-rev

CGGAATTCATAAGTGAGAGCTGTAACTCTCGC

PFur-for

CAGCTGCTGGCTGATGATGACCACTTTGT

PFur-rev

CGGAATTCATAAGTGAGAGCTGTAACTCTCGC

E-for

CGCGGATCCATGGTACGCTGGACTTTGTGGGA

E-rev

AAAACTGCAGTCACTCCTTCCGCACGTAATTTT

gef-for

CGCGGATCCATGAAGCAGCATAAGGCGATGATTG

gef-rev

AAAACTGCAGTTACTCGGATTCGTAAGCCGTGAAA

mazF-for

CGCGGATCCATGGTAAGCCGATACGTACCCGATAT

mazF-rev

AAAACTGCAGCTACCCAATCAGTACGTTAATTTT

  1. a Restriction enzyme sites used for cloning of PCR products are underlined.