Strategy | Modified strategies and descriptions | Repression RF | Induction RF | Induction ratio | Relative induction fold |
---|---|---|---|---|---|
NC | E. coli Top10 | 200 ± 10 | 200 ± 9 | 0 | 1 |
PC | PBAD | 260 ± 11 | 7812 ± 31 | 126 | 30 |
0 | Original fhuA promoter | 3292 ± 20 | 10116 ± 37 | 3 | 51 |
1 | Changed the Fur box in −10 region into a conserved Fur box from E. coli[17] | 250 ± 12 | 5466 ± 26 | 101 | 27 |
2 | Modified the Fur box in −10 region into an enhanced Fur box [17] | 196 ± 5 | 1885 ± 17 | BDL | 9 |
3 | Integrated a designed Fur box in −35 region | 200 ± 9 | 662 ± 19 | BDL | 3 |
4 | Inserted the SD-fur-TT circuit in the vector | 230 ± 9 | 3464 ± 24 | 108 | 17 |
5 | Inserted the Pfur-SDfur-TT circuit in the vector | 195 ± 7 | 2389 ± 26 | BDL | 12 |