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Table 3 Resistance and enzyme activity profiles of S. venezuelae strains

From: Streptomyces spp. as efficient expression system for a d,d-peptidase/d,d-carboxypeptidase involved in glycopeptide antibiotic resistance

  MIC Enzyme activity
Strain Vancomycin (μg/mL) Teicoplanin (μg/mL) d,d-carboxypeptidase (U/mg) d,d-peptidase (U/mg)
wild-type 0.2 0.5 0 0
pIJ86 0.2 0.5 0 0
pIJ86ΩC-His6-vanY n 0.4 0.7 189 ± 5.5 100 ± 3.9
pIJ86ΩN-His6-vanY n 0.2 0.5 0 0
pIJ86ΩvanY n 0.4 0.7 172 ± 4.3 80 ± 3.2
  1. MICs of glycopeptides were determined by plating 106 cfu/mL of each strain on agar medium added with different antibiotic concentrations. Results are the average of three independent experiments in which the standard deviation was less than 5%. Specific activity (U/mg protein) was determined in alkaline cell extracts added to 10 mM solutions of acetyl-l-Lys-d-Ala-d-Ala or d-Ala-d-Ala. The activity was assayed at 25°C as described in the Methods section [17]. Results are the average of three independent experiments.
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