| |
MIC
|
Enzyme activity
|
|---|
|
Strain
|
Vancomycin (μg/mL)
|
Teicoplanin (μg/mL)
|
d,d-carboxypeptidase (U/mg)
|
d,d-peptidase (U/mg)
|
|---|
|
wild-type
|
0.2
|
0.5
|
0
|
0
|
|
pIJ86
|
0.2
|
0.5
|
0
|
0
|
|
pIJ86ΩC-His6-vanY
n
|
0.4
|
0.7
|
189 ± 5.5
|
100 ± 3.9
|
|
pIJ86ΩN-His6-vanY
n
|
0.2
|
0.5
|
0
|
0
|
|
pIJ86ΩvanY
n
|
0.4
|
0.7
|
172 ± 4.3
|
80 ± 3.2
|
- MICs of glycopeptides were determined by plating 106 cfu/mL of each strain on agar medium added with different antibiotic concentrations. Results are the average of three independent experiments in which the standard deviation was less than 5%. Specific activity (U/mg protein) was determined in alkaline cell extracts added to 10 mM solutions of acetyl-l-Lys-d-Ala-d-Ala or d-Ala-d-Ala. The activity was assayed at 25°C as described in the Methods section [17]. Results are the average of three independent experiments.
