|
S. venezuelae
|
E. coli
|
---|
Substrate
|
C-His6-VanYn(U/mg)
|
N-His6−VanYn(U/mg)
|
C-His6−VanYn (U/mg)
|
---|
d-Ala-d-Ala
|
18 ± 5.0
|
0
|
19 ± 3
|
Nα,Nε-diacetyl-l-Lys-d-Ala-d-Ala
|
38 ± 3.8
|
0
|
36 ± 4.0
|
Acetyl-l-Lys-d-Ala-d-Ala
|
40 ± 5.6
|
0
|
40 ± 3.0
|
- Specific activity (U/mg protein) was determined using 40 μg of His6-VanYn added to 10 mM solutions of the indicated compounds. The activity was assayed at 25°C as described in the Methods section. Results are the average of three independent experiments.