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Table 2 Substrate specificity of His 6 -VanY n recombinant forms produced by different hosts

From: Streptomyces spp. as efficient expression system for a d,d-peptidase/d,d-carboxypeptidase involved in glycopeptide antibiotic resistance

 

S. venezuelae

E. coli

Substrate

C-His6-VanYn(U/mg)

N-His6−VanYn(U/mg)

C-His6−VanYn (U/mg)

d-Ala-d-Ala

18 ± 5.0

0

19 ± 3

Nα,Nε-diacetyl-l-Lys-d-Ala-d-Ala

38 ± 3.8

0

36 ± 4.0

Acetyl-l-Lys-d-Ala-d-Ala

40 ± 5.6

0

40 ± 3.0

  1. Specific activity (U/mg protein) was determined using 40 μg of His6-VanYn added to 10 mM solutions of the indicated compounds. The activity was assayed at 25°C as described in the Methods section. Results are the average of three independent experiments.
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BMC Biotechnology

ISSN: 1472-6750

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