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Table 1 Biomass and VanY n production in recombinant Streptomyces spp.

From: Streptomyces spp. as efficient expression system for a d,d-peptidase/d,d-carboxypeptidase involved in glycopeptide antibiotic resistance

Strain Vector Medium Dry weight (g cell/L) VanYn(mg/g cell) VanYn(mg/L)
S. venezuelae pIJ86ΩC-His6-vanY n BTSB 30 0.94 28.4
S. venezuelae pIJ86ΩC-His6-vanY n YEME 30 0.97 30.7
S. venezuelae pIJ86ΩN-His6-vanY n BTSB 36 1.47 23.9
S. venezuelae pIJ86ΩN-His6-vanY n YEME 35 1.10 31.6
S. coelicolor ΔvanRS pIJ86ΩC-His6-vanY n BTSB 25 1.16 28.9
S. coelicolor ΔvanRS pIJ86ΩC-His6-vanY n YEME 20 0.62 12.4
S. coelicolor ΔvanRS pIJ86ΩN-His6-vanY n BTSB 30 1.06 31.8
S. coelicolor ΔvanRS pIJ86ΩN-His6-vanY n YEME 8 0.79 6.3
S. lividans pIJ86ΩC-His6-vanY n BTSB 20 1.02 20.3
S. lividans pIJ86ΩN-His6-vanY n BTSB 22 0.80 18
  1. Streptomyces spp. recombinant strains carrying different constructs (C-His6-vanY n or N-His6-vanY n ) were grown in YEME or BTSB for 72 hours. VanYn was quantified by immunoblotting on crude extracts as reported in the Methods.
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BMC Biotechnology

ISSN: 1472-6750

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