Strain
|
Vector
|
Medium
|
Dry weight (g cell/L)
|
VanYn(mg/g cell)
|
VanYn(mg/L)
|
---|
S. venezuelae
|
pIJ86ΩC-His6-vanY
n
|
BTSB
|
30
|
0.94
|
28.4
|
S. venezuelae
|
pIJ86ΩC-His6-vanY
n
|
YEME
|
30
|
0.97
|
30.7
|
S. venezuelae
|
pIJ86ΩN-His6-vanY
n
|
BTSB
|
36
|
1.47
|
23.9
|
S. venezuelae
|
pIJ86ΩN-His6-vanY
n
|
YEME
|
35
|
1.10
|
31.6
|
S. coelicolor ΔvanRS
|
pIJ86ΩC-His6-vanY
n
|
BTSB
|
25
|
1.16
|
28.9
|
S. coelicolor ΔvanRS
|
pIJ86ΩC-His6-vanY
n
|
YEME
|
20
|
0.62
|
12.4
|
S. coelicolor ΔvanRS
|
pIJ86ΩN-His6-vanY
n
|
BTSB
|
30
|
1.06
|
31.8
|
S. coelicolor ΔvanRS
|
pIJ86ΩN-His6-vanY
n
|
YEME
|
8
|
0.79
|
6.3
|
S. lividans
|
pIJ86ΩC-His6-vanY
n
|
BTSB
|
20
|
1.02
|
20.3
|
S. lividans
|
pIJ86ΩN-His6-vanY
n
|
BTSB
|
22
|
0.80
|
18
|
- Streptomyces spp. recombinant strains carrying different constructs (C-His6-vanY
n
or N-His6-vanY
n
) were grown in YEME or BTSB for 72 hours. VanYn was quantified by immunoblotting on crude extracts as reported in the Methods.