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Table 1 Biomass and VanY n production in recombinant Streptomyces spp.

From: Streptomyces spp. as efficient expression system for a d,d-peptidase/d,d-carboxypeptidase involved in glycopeptide antibiotic resistance

Strain

Vector

Medium

Dry weight (g cell/L)

VanYn(mg/g cell)

VanYn(mg/L)

S. venezuelae

pIJ86ΩC-His6-vanY n

BTSB

30

0.94

28.4

S. venezuelae

pIJ86ΩC-His6-vanY n

YEME

30

0.97

30.7

S. venezuelae

pIJ86ΩN-His6-vanY n

BTSB

36

1.47

23.9

S. venezuelae

pIJ86ΩN-His6-vanY n

YEME

35

1.10

31.6

S. coelicolor ΔvanRS

pIJ86ΩC-His6-vanY n

BTSB

25

1.16

28.9

S. coelicolor ΔvanRS

pIJ86ΩC-His6-vanY n

YEME

20

0.62

12.4

S. coelicolor ΔvanRS

pIJ86ΩN-His6-vanY n

BTSB

30

1.06

31.8

S. coelicolor ΔvanRS

pIJ86ΩN-His6-vanY n

YEME

8

0.79

6.3

S. lividans

pIJ86ΩC-His6-vanY n

BTSB

20

1.02

20.3

S. lividans

pIJ86ΩN-His6-vanY n

BTSB

22

0.80

18

  1. Streptomyces spp. recombinant strains carrying different constructs (C-His6-vanY n or N-His6-vanY n ) were grown in YEME or BTSB for 72 hours. VanYn was quantified by immunoblotting on crude extracts as reported in the Methods.