Figure 3

Growth curve and VanY _n production in 3-L batch fermentations of S. venezuelae pIJ86ΩC-His ₆ - vanY _n in YEME. (A) Time courses of pH (□, dashed line), pO₂ (●, solid line), glucose (○, dashed line), and growth curve measured as dry weight (♦, solid line). (B) Time course of recombinant VanY_n production as determined by quantitative Western blot analysis of crude extracts from cell samples collected at different times of fermentation (see panel C). Maximum protein expression at 48 hours of growth was set as 100% (corresponding to ~ 30 mg/L of culture). Total protein content in each fermentation sample was determined by Biuret method (● , solid line). In (C), samples corresponding to 100 μL of cell culture were loaded in each lane; Std, standard reference protein: C-His₆-VanY_n from E. coli (5 μg, 25 kDa). The corresponding SDS-PAGE is shown in the Additional file 2: Figure S2.