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Table 6 The effect of selected reagents on BglMKg β-glucosidase activity

From: Cloning and characterization of a novel cold-active glycoside hydrolase family 1 enzyme with β-glucosidase, β-fucosidase and β-galactosidase activities

Reagents

Relative activity (%)

 

10 mM

100 mM

Control

100

100

EDTA

97

81

SDS

< 0.01

< 0.01

Urea

100

95

DTT

110

153

Glutathione oxidized

2

< 0.01

2-mercaptoethanol

98

92

Hydrochloride guanidine

89

58

Tris

41

< 0.01

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BMC Biotechnology

ISSN: 1472-6750

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