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Table 1 Purification of the recombinant BglMKg (U for β-galactosidase activity)

From: Cloning and characterization of a novel cold-active glycoside hydrolase family 1 enzyme with β-glucosidase, β-fucosidase and β-galactosidase activities

Step

Total protein (mg)

Total activity (U)

Specific activity (U/mg)

Purification (fold)

Yield (%)

Crude extract

434.5

759

1.75

1.0

100

Fractogel EMD DEAE

72.0

302

4.19

2.4

40