Figure 2From: A mutant screening method by critical annealing temperature-PCR for site-directed mutagenesisT c -PCR screening and sequence analysis on 16 clones of D112K and D112G after SDM. The Tc is 66°C for D112K and 68°C D112G. The sequencing results are represented by M (desired mutant), W (wild type) and U (undesired mutant), respectively. N.C and W.C represent negative and WT controls, respectively.Back to article page