Figure 5From: Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensisin detergents and its ability to degrade the abnormal prion proteinWestern blots of PrPScTk-SP digests. 1% MBH was subjected to digestion at 100°C for 1 h with buffer only (lane 1), 0.02 mg/ml (0.4 mM) Tk-SP (lane 2), 1% SDS (lane 3), or 0.02 mg/ml (0.4 mM) Tk-SP plus 1% SDS (lane 4). The treated samples were analyzed by western blot using mAb SAF-83. Lane M, marker.Back to article page