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Figure 4 | BMC Biotechnology

Figure 4

From: Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensisin detergents and its ability to degrade the abnormal prion protein

Figure 4

Effect of 0.05% (w/v) nonionic surfactants and 0.01% (w/v) EDTA on the activity of Tk-SP. Tk-SP was incubated at 80°C for 20 min in 50 mM Tris–HCl (pH 7.5) containing the nonionic surfactants indicated, and the relative activities were determined using azocasein as the substrate. Each experiment was carried out three times, and the average values are shown together with the error bars. Lane 1, EMULGEN 147; lane 2, EMULGEN LS-114; lane 3, EMULGEN PP-290; lane 4, RHEODOL Tw-0120 V.

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BMC Biotechnology

ISSN: 1472-6750

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