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Table 5 Summary of the optimized process used in this study for tyrosinase production

From: High level production of tyrosinase in recombinant Escherichia coli

Parameters Tyrosinase production
Strain JM109 (pMFvpt)
Medium Minimal medium containing glycerol and NZ-amine
Temperature (°C) 32
pH 6.9
Dissolved oxygen (%) 30
Cultivation Fed-batch culture with exponential feeding
Feeding medium Glycerol and NZ-amine
Feeding rate (h-1) 0.3
Induction stage At OD600 value of 30-40
Harvest End of the exponential growth