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Table 3 Influence of culture temperature on tyrosinase production

From: High level production of tyrosinase in recombinant Escherichia coli

Temperature μmax(h-1) a OD600maxb Max. act. (U mL-1) c
25 (°C) 0.31 ± 0.02 7.8 ± 0.9 0.12 ± 0.03
30 (°C) 0.52 ± 0.02 8.0 ± 0.7 0.22 ± 0.03
32 (°C) 0.60 ± 0.03 10.2 ± 0.9 0.76 ± 0.06
37 (°C) 0.66 ± 0.02 10.8 ± 1.0 0.36 ± 0.05
  1. a. μmax: the maximal growth rate.
  2. b. OD600max: the maximal optical density measured at 600 nm.
  3. c. Max. act.: the maximal volumetric activity of tyrosinase in units per mL of culture broth.
  4. The freshly transformed JM109 (pMFvpt) cells were grown in shake flasks at 25, 30, 32 or 37°C and induced at the early-exponential growth phase (OD600 ≈ 1.1) with 1.0 mM IPTG.