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Table 1 Comparison of different E. coli hosts for tyrosinase production

From: High level production of tyrosinase in recombinant Escherichia coli

Hosts a μmax(h-1) b OD600maxc Max. act. (U mL-1) d
DH5α 0.26 ± 0.04 7.7 ± 0.4 0.44 ± 0.05
DH5α + IPTG 0.27 ± 0.03 8.0 ± 0.3 0.44 ± 0.01
BLR 0.60 ± 0.01 8.9 ± 0.4 0.03 ± 0.00
BLR + IPTG 0.57 ± 0.02 7.5 ± 0.6 0.03 ± 0.00
JM109 0.60 ± 0.02 7.4 ± 0.3 0.03 ± 0.00
JM109 + IPTG 0.59 ± 0.03 7.7 ± 0.4 0.07 ± 0.01
  1. a. Cells were grown in shake flasks at 37°C with or without 1 mM IPTG induction at the early growth phase (OD600 ≈ 0.6).
  2. b. μmax: the maximal growth rate.
  3. c. OD600max: the maximal optical density measured at 600 nm.
  4. d. Max. act.: the maximal volumetric activity of tyrosinase in units per mL of culture broth.