Dispase dissociation and cytoskeletal disruption alter cytoskeletal distribution. Cytoskeletal labeling of (A) actin, (B) microtubules and (C) keratin intermediate filaments in keratinocytes under conditions of control, lifted, toxin treatment, and lifting + toxin treatment. The keratin stain using Monoclonal Anti-Cytokeratin, pan antibody was very dense and thus individual filaments are not visible easily. Scale bar is 10 μm. (D) Whole sheet images in 35 mm dishes and cross-sectional thickness images under conditions of control, dispase-lifted, toxin treatment, and lifting + toxin treatment. Cells were stained with CellTracker Green. Scale bar is 50 μm.