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Table 2 Purification of the r Aci HBGase II-(His) 6 enzyme

From: Expression of a secretory α-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization

Procedure

TotalPrProtein (mg)

Total activity (U)

Specific activity (mU/mg)a

Yield (%)

Purification fold

Negative control (Empty vector used)

3720

1.0

0.26

-

-

Supernatant (100 ml)

2326

2.6

0.11

100

1.0

Concentrated with Viva spin 20

151.5

0.20

1.3

7.69

1.20

Histrap column chromatography

11.25

0.018

1.6

0.69

1.45

  1. a mU = 10-3 U.
  2. The rAciHBGase II-(His)6 enzyme was partially purified from 100 ml of a 1.2 liter culture of transformed P. pastoris after 96 h induction with 0.5% (v/v) methanol.
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BMC Biotechnology

ISSN: 1472-6750

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