Table 1 Primers used for the RT-PCR to obtain the full-length cDNA of AciHBGase II
Primer name | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Ta (oC)a | Size (bp)b |
|---|---|---|---|---|
Pair 3_HBGase I | AATGGCGAGAATTTTGTGGAC | TGGAGTTTACGCTGCTTGTG | 53 | 783 |
Pair 1_HBGase II | ATGTTTCGAGCGACGATAGTTAC | AAGACGCTGAGCCAATTGTT | 52 | 503 |
Pair 2_HBGase II | CGAGGAGTTTCCAAGACAGC | CTCGAACATGTGGTTGATGG | 42 | 581 |
Pair 3_HBGase II | AGTACTACGTGTGGCGGGAC | GGACTTGAACGCCACGTAAT | 52 | 983 |
Pair 4_HBGase II | CGTGATGCTGACGTTGACTT | TTACAACCAGTCTACACCTTGCC | 48 | 634 |
28S rDNA | AAAGATCGAATGGGGATATTC | CACCGGGTCCGTACCTCC | 44 | 350 |