Figure 4
Enrichment profile for the r Aci HBGase II–(His) 6 protein by Histrap affinity column. (A) Elution profile from the Histrap column. The washed fractions contained a high content of protein while eluted fraction # 10 presented the highest specific activity of HBGase II. (B) SDS-PAGE resolved and coomassie blue stained protein profile of selected fractions from the Histrap column elution. Lane 1: standard protein marker, lanes 2-6: contained 1 μg of protein in fractions # 2, 4, 6, 8 and 10, respectively. Lane 7 is a duplication of lane 6. (C) Glucosidase activity stain of the renatured SDS-PAGE resolved protein samples (Zymograph). Lanes 1-3, fractions # 9-11, respectively. Arrows in (B) and (C) indicate the ~ 73 kDa band.