Discovery and characterization of a highly efficient enantioselective mandelonitrile hydrolase from Burkholderia cenocepacia J2315 by phylogeny-based enzymatic substrate specificity prediction

Table 4 Kinetic parameters, specific activity, enantioselectivity, and specificity of BCJ2315 compared with other mandelonitrile hydrolases[33–35]

Organism	Kinetic parameters		Specific activity (U/mg)	ee value of acid (%)	Amide formation (% of total products)	Reference
Organism	K_m (mM)	V_max (μmol/min/mg)	Specific activity (U/mg)	ee value of acid (%)	Amide formation (% of total products)	Reference
uncultured organism ^a	N.A.	N.A.	50	98	N.D.	[34]
Pseudomonas fluorescens EBC191	N.A.	N.A.	32.8	31	19	[19]
Burkholderia cenocepacia J2315	0.14	45.4	27.79	98.4	N.D.	This study
Bradyrhizobium japonicum USDA110^b	0.26	44.7	24.38	-	N.D.	[21]
Alcaligenes sp. ECU0401	21.8	27.9	19	97	N.D.	[11]
Alcaligenes faecalis JM3	N.A.	N.A.	12.4	99	N.D.	[13]
Aspergillus niger CBS 513.88	11.4	12.4	7.7	99	N.D.	[16], [35]
Neurospora crassa OR74A	3.4	9.9	6.1	99	15	[16], [35]
Pseudomonas putida	13.39	16.5	3.26	99.9	N.D.	[33]
Alcaligenes faecalis ATCC8750	N.A.	N.A.	3.1	100	N.D.	[10]
Burkholderia xenovorans LB400	0.084	N.A.	1.523	-	N.D.	[22]
Alcaligenes faecalis ZJUTB10	4.74	15.85	N.A.	99	N.D.	[12]

ISSN: 1472-6750