Discovery and characterization of a highly efficient enantioselective mandelonitrile hydrolase from Burkholderia cenocepacia J2315 by phylogeny-based enzymatic substrate specificity prediction

Table 3 Substrate specificity of the purified BCJ2315

Entry	Substrate	Relative activity^a (%)
1	Iminodiacetonitrile	21±1
2	acrylonitrile	17±1
3	2-Methylglutaronitrile	25±3
4	Succinonitrile	5±1
5	Fumaronitrile	78±3
6	Malononitrile	17±1
7	Valeronitrile	4±1
8	3-Hydroxyglutaronitrile	N.D.
9	4-Chlorobutyronitrile	11±1
10	Glycolonitrile	69±4
11	3-Phenylpropionitrile	N.D.
12	Benzonitrile	N.D.
13	Cinnamonitrile	N.D.
14	Phenylacetonitrile	100±4
15	2-Chloromandelonitrile	298±7
16	1,2-Phenylenediacetonitrile	37±5
17	2-Phenylbutyronitrile	N.D.
18	alpha-Methylphenylacetonitrile	N.D.
19	mandelonitrile	884±7
20	3-Cyanopyridine	N.D.
21	Thiophene-3-carbonitrile	1±1
22	Indole-3-acetonitrile	20±1
23	2-Cyanopyridine	7±1
24	4-Cyanopyridine	7±1

^a Nitrilase activity against various nitriles was measured under standard assay conditions. The activity toward phenylacetonitrile was defined as 100%.
N.D. not detected.

ISSN: 1472-6750