Skip to main content

Table 1 Effect of organic solvents on BioHx stability after incubation at 30°C for 1 h

From: Molecular cloning of a novel bioH gene from an environmental metagenome encoding a carboxylesterase with exceptional tolerance to organic solvents

Organic solvents

Residual activity (%)

20%

30%

40%

50%

Control

100 ± 0.10

100 ± 0.00

100 ± 0.00

100 ± 0.00

Methanol

94.6 ± 3.2

93.6 ± 4.4

85.5 ± 2.3

57.0 ± 4.0

Ethanol

85.5 ± 0.8

82.2 ± 3.2

77.9 ± 2.1

75.4 ± 4.1

2-Propanol

86.3 ± 3.2

86.3 ± 4.5

76.8 ± 4.4

76.2 ± 3.0

1-Butanol

48.9 ± 3.6

47.9 ± 3.0

30.8 ± 2.7

23.5 ± 2.2

Acetonitrile

74.4 ± 1.2

74.4 ± 3.3

65.0 ± 4.3

44.3 ± 4.5

Acetone

79.6 ± 1.9

78.0 ± 2.7

62.1 ± 3.7

15.9 ± 4.4

DMSO

95.7 ± 2.4

95.3 ± 1.9

54.2 ± 1.0

29.7 ± 3.8

DMF

97.7 ± 0.8

97.3 ± 1.3

72.3 ± 2.1

19.7 ± 1.1

  1. No organic solvent was included in the control samples. The concentrations of organic solvents are indicated (v/v). The enzyme activity was determined under the standard assay condition, and the data is represented as an average of three independent determinations.
Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us