Figure 7From: Molecular cloning of a novel bioH gene from an environmental metagenome encoding a carboxylesterase with exceptional tolerance to organic solventsEffect of metal ions and detergents on BioHx Stability. Aliquots of the enzyme were incubated with each metal ion (10 mM) and detergent (0.5%) at 30°C for 1 h, the residual activity of BioHx was determined using pNP-butyrate as the substrate. All determinants were performed in triplicate.Back to article page