Skip to main content

Table 2 Primer for vector amplification and complementary primer extensions for gene of interest

From: A new method to customize protein expression vectors for fast, efficient and background free parallel cloning

LP1 forward vector primer

3C

5' GGGCCCCTGGAACAGAACTTCCAG 3'

Sumo1

5' TCCACCGGTTTGTTCCTGGTAGAC 3'

Sumo3

5' TCCACCGGTCTGCTGCTGGAACAC 3'

N-tagless pET

5' GGTATATCTCCTTCTCTAGAGGGGAATTGTTATCCGCTC 3'

N-tagless pFastBac

5' GGAATTCCGCGCGCTTCGGACC 3'

LP2 reverse vector primer

 

ccdB

5' CGCCATTAACCTGATGTTCTGGGG 3'

10His

5' GAGCATCATCATCATCACCAC 3'

OneStrep

5' AGCGCTTGGAGCCACCCGCAG 3'

S

5' AAAGAAACCGCTGCTGCTAAATTCG 3'

HPC4

5' GAGGACCAGGTGGACCCCCGG 3'

LP1 forward gene primer

 

3C

5' AAGTTCTGTTCCAGGGGCCC – GOI seq 3'

Sumo1

5' GTCTACCAGGAACAAACCGGTGGA – GOI seq 3'

Sumo3

5' GTGTTCCAGCAGCAGACCGGTGGA – GOI seq 3'

LP2 reverse gene primer

 

ccdB

5' CCCCAGAACATCAGGTTAATGGCG - Stop - GOI - seq 3'

10His

5' GTGGTGATGATGATGATGCTC - Stop - GOI - seq 3'

OneStrep

5' CTGCGGGTGGCTCCAAGCGCT - Stop - GOI - seq 3'

S

5' AGCAGCAGCGGTTTCTTT - Stop - GOI - seq 3'

CBP

5' CTTCCACCGCCGCTTCATC - Stop - GOI - seq 3'

HPC4

5' GGGGTCCACCTGGTCCTC – Stop - GOI - seq 3'

Æ54CPD54

5' CAGGATCTTGCCGCTGCC – Stop - GOI - seq 3'