A new method to customize protein expression vectors for fast, efficient and background free parallel cloning

BMC Biotechnology

Table 1 Current list of pCoofys

pCoofy	parental vector	N-tag	C-tag	Host	fd primer	rev primer
pCoofy1	pETM14	His6	none	E. coli	3C	ccdB
pCoofy18	pCoofy1	His10	none	E. coli	3C	ccdB
pCoofy7	pETM14	S	none	E. coli	3C	ccdB
pCoofy12	pCoofy1	OneStrep	none	E. coli	3C	ccdB
pCoofy34	pCoofy12	S-OneStrep	none	E. coli	3C	ccdB
pCoofy21	pCoofy12	His10-OneStrep	none	E. coli	3C	ccdB
pCoofy19	pCoofy1	CBP	none	E. coli	3C	ccdB
pCoofy2	pETM22	Trx-His6	none	E. coli	3C	ccdB
pCoofy38	pCoofy2	Trx-His10	none	E. coli	3C	ccdB
pCoofy3	pETM33	His6-GST	none	E. coli	3C	ccdB
pCoofy8	pETM14	Halo	none	E. coli	3C	ccdB
pCoofy35	pCoofy4	MBP	none	E. coli	3C	ccdB
pCoofy4	pETM44	His6-MBP	none	E. coli	3C	ccdB
pCoofy15	pETM14	NusA	none	E. coli	3C	ccdB
pCoofy16	pCoofy15	His10-NusA	none	E. coli	3C	ccdB
pCoofy5	pET28M-Sumo1	His6-Sumo1	none	E. coli	Sumo1	ccdB
pCoofy6	pET28M-Sumo3	His6-Sumo3	none	E. coli	Sumo3	ccdB
pCoofy17	pCoofy6	His10-Sumo3	none	E. coli	Sumo3	ccdB
pCoofy22	pCoofy7	S	His10	E. coli	3C	10His
pCoofy31	pCoofy12	OneStrep	His10	E. coli	3C	10His
pCoofy36	pCoofy34	S-OneStrep	His10	E. coli	3C	10His
pCoofy24	pCoofy19	CBP	His10	E. coli	3C	10His
pCoofy23	pCoofy14	Trx	His10	E. coli	3C	10His
pCoofy25	pCoofy8	Halo	His10	E. coli	3C	10His
pCoofy37	pCoofy35	MBP	His10	E. coli	3C	10His
pCoofy26	pCoofy15	NusA	His10	E. coli	3C	10His
pCoofy32	pCoofy1	His6	OneStrep	E. coli	3C	OneStrep
pCoofy33	pCoofy18	His10	OneStrep	E. coli	3C	OneStrep
pCoofy11	pIEX1	His10	none	Insect	3C	ccdB
pCoofy27	pFastBac	His6	none	Insect	3C	ccdB
pCoofy28	pFastBac	His6-GST	none	Insect	3C	ccdB
pCoofy29	pFastBac	His6-MBP	none	Insect	3C	ccdB
pCoofy40	pTT5	none	flexible	HEK293E	3C	flexible

ISSN: 1472-6750