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Table 1 Current list of pCoofys

From: A new method to customize protein expression vectors for fast, efficient and background free parallel cloning

pCoofy parental vector N-tag C-tag Host fd primer rev primer
pCoofy1 pETM14 His6 none E. coli 3C ccdB
pCoofy18 pCoofy1 His10 none E. coli 3C ccdB
pCoofy7 pETM14 S none E. coli 3C ccdB
pCoofy12 pCoofy1 OneStrep none E. coli 3C ccdB
pCoofy34 pCoofy12 S-OneStrep none E. coli 3C ccdB
pCoofy21 pCoofy12 His10-OneStrep none E. coli 3C ccdB
pCoofy19 pCoofy1 CBP none E. coli 3C ccdB
pCoofy2 pETM22 Trx-His6 none E. coli 3C ccdB
pCoofy38 pCoofy2 Trx-His10 none E. coli 3C ccdB
pCoofy3 pETM33 His6-GST none E. coli 3C ccdB
pCoofy8 pETM14 Halo none E. coli 3C ccdB
pCoofy35 pCoofy4 MBP none E. coli 3C ccdB
pCoofy4 pETM44 His6-MBP none E. coli 3C ccdB
pCoofy15 pETM14 NusA none E. coli 3C ccdB
pCoofy16 pCoofy15 His10-NusA none E. coli 3C ccdB
pCoofy5 pET28M-Sumo1 His6-Sumo1 none E. coli Sumo1 ccdB
pCoofy6 pET28M-Sumo3 His6-Sumo3 none E. coli Sumo3 ccdB
pCoofy17 pCoofy6 His10-Sumo3 none E. coli Sumo3 ccdB
pCoofy22 pCoofy7 S His10 E. coli 3C 10His
pCoofy31 pCoofy12 OneStrep His10 E. coli 3C 10His
pCoofy36 pCoofy34 S-OneStrep His10 E. coli 3C 10His
pCoofy24 pCoofy19 CBP His10 E. coli 3C 10His
pCoofy23 pCoofy14 Trx His10 E. coli 3C 10His
pCoofy25 pCoofy8 Halo His10 E. coli 3C 10His
pCoofy37 pCoofy35 MBP His10 E. coli 3C 10His
pCoofy26 pCoofy15 NusA His10 E. coli 3C 10His
pCoofy32 pCoofy1 His6 OneStrep E. coli 3C OneStrep
pCoofy33 pCoofy18 His10 OneStrep E. coli 3C OneStrep
pCoofy11 pIEX1 His10 none Insect 3C ccdB
pCoofy27 pFastBac His6 none Insect 3C ccdB
pCoofy28 pFastBac His6-GST none Insect 3C ccdB
pCoofy29 pFastBac His6-MBP none Insect 3C ccdB
pCoofy40 pTT5 none flexible HEK293E 3C flexible