Table 2 Oligonucleotides used in this study
From: An improved genetic system for bioengineering buoyant gas vesicle nanoparticles from Haloarchaea
Oligonucleotide | 5′-3′ sequence | Use |
|---|---|---|
gvpCdel5F | CGCAAGCTTATTACTTCTCTCCAGTCGATG | gvpC deletion construction |
gvpCdel5R | GCGGGCAGTACTCATCTCGTCCTCGAGGCGTTTGTCTGTGACACTCAT | |
gvpCdel3F | GACGAGATGAGTACTGCCCGCCGGCCGGATGATAAAACATGA | |
gvpCdel3R | CGCAAGCTTACTCGTTGTAGACCAGCGTTG | |
gvpA-NdeI | CTCAAGGTATACCACTAGACCCTAAT | Amplification of gvpA promoter |
gvpA-KpnI | ACTCATGGTACCTACTTCTCTCCAGT | |
gvpC-F1AflIIGvpC-F | GGTGTGCTTAAGATGAGTGTCACAGACAAA | gvpC gene segment amplification |
gvpC-C1RAvrIIGvpC | CAGCCTAGGGTGGGTTGAGTTCATCTCTGT | |
gvpC-C2RAvrIIGvpC | CTGCCTAGGCGCGGTAGCGTCGAAGCTGTC | |
gvpC-C3RAvrIIGvpC | GTGCCTAGGTTCTGCTTCCGCTTCGAC | |
gvpC-C4RAvrIIGvpC | AGACCTAGGTGTTTTATCATCCGGCCG | |
gvpC-His-adapter F | CGTCTCCATATGCACCACCACCACCACCACCTTAAGCGTCTACCTAGGAGCGCTTGAGGATCCATC | His-tag adapter for gvpC and antigen fusion expression plasmid construction |
gvpC-His-adapter R | GATGGATCCTCAAGCGCTCCTAGGTAGACGCTTAAGGTGGTGGTGGTGGTGGTGCATATGGAGACG | |
pKJ-cspD2F | GCTGGACTGCCTTTTCTTCG | Sequencing of promoters and inserts in pARK and pDRK series plasmids |
pKJ-BamH1-3′160R | GTTACTCCACCGTCATTCAG | |
Universal F 20mer | GTTGTAAAACGACGGCCAGT | Sequencing across gvpC deletion |
Universal R 20mer | CACAGGAAACAGCTATGACC | |
Luci Int R | GTGGCTGAGGCAGATGAGGC | Sequencing and determination of luciferase gene orientation |