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Table 1 Strains and plasmids used in this study

From: An improved genetic system for bioengineering buoyant gas vesicle nanoparticles from Haloarchaea

Strain/plasmid Characteristics Source or reference
Halobacterium sp. strain NRC-1 Sequenced wild-type strain Laboratory collection [28]
Halobacterium sp. strain SD109 Strain NRC-1 deleted for the gvp gene cluster of pNRC100 Laboratory collection [13]
Halobacterium sp. strain NRC-1Δura3 Strain NRC-1 deleted for ura3 gene coding orotidine-5′-monophosphate Laboratory collection [2427]
Halobacterium sp. strain NRC-1ΔuragvpC Strain NRC-1Δura3 deleted for the gvpC gene This study
pFM104gvpC::κ1 Halobacterium-E. coli shuttle plasmid containing entire gvp gene cluster with κ insertion in the gvpC gene Laboratory collection [11]
pBB400 Suicide plasmid capable of replication in E. coli but not Halobacterium, containing the ura3 gene Laboratory collection [26]
pBB400ΔgvpC pBB400 plasmid containing gvpC gene-flanking regions for deletion construction This study
pMC2 Halobacterium expression vector with cspD2 promoter and Halorubrum lacusprofundi β-galactosidase gene Laboratory collection [27]
pARK Halobacterium sp. NRC-1 expression vector with cspD2 promoter and adapter containing a start codon, His-tag, and restriction sites for insertion of the gvpC gene fragments This study
pARK-C1 pARK derivative with gvpC gene C1 fragment This study
pARK-C2 pARK derivative with gvpC gene C2 fragment This study
pARK-C3 pARK derivative with gvpC gene C3 fragment This study
pARK-C4 pARK derivative with gvpC complete gene (C4) This study
pDRK Halobacterium sp. NRC-1 expression vector with gvpA promoter and adapter containing a start codon, His-tag, and restriction sites used for insertion of the gvpC gene fragments This study
pDRK-C1-L pDRK derivative containing gvpC gene C1 fragment fused to codon-optimized Gaussia princeps luciferase gene This study
pDRK-C2-L pDRK derivative containing gvpC gene C2 fragment fused to codon-optimized Gaussia princeps luciferase gene This study
pDRK-C3-L pDRK derivative containing gvpC gene C3 fragment fused to codon-optimized Gaussia princeps luciferase gene This study
pDRK-C4-L pDRK derivative containing gvpC complete gene (C4) fused to codon-optimized Gaussia princeps luciferase gene This study