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Table 1 Strains and plasmids used in this study

From: An improved genetic system for bioengineering buoyant gas vesicle nanoparticles from Haloarchaea

Strain/plasmid

Characteristics

Source or reference

Halobacterium sp. strain NRC-1

Sequenced wild-type strain

Laboratory collection [28]

Halobacterium sp. strain SD109

Strain NRC-1 deleted for the gvp gene cluster of pNRC100

Laboratory collection [13]

Halobacterium sp. strain NRC-1Δura3

Strain NRC-1 deleted for ura3 gene coding orotidine-5′-monophosphate

Laboratory collection [24–27]

Halobacterium sp. strain NRC-1Δura3ΔgvpC

Strain NRC-1Δura3 deleted for the gvpC gene

This study

pFM104gvpC::κ1

Halobacterium-E. coli shuttle plasmid containing entire gvp gene cluster with κ insertion in the gvpC gene

Laboratory collection [11]

pBB400

Suicide plasmid capable of replication in E. coli but not Halobacterium, containing the ura3 gene

Laboratory collection [26]

pBB400ΔgvpC

pBB400 plasmid containing gvpC gene-flanking regions for deletion construction

This study

pMC2

Halobacterium expression vector with cspD2 promoter and Halorubrum lacusprofundi β-galactosidase gene

Laboratory collection [27]

pARK

Halobacterium sp. NRC-1 expression vector with cspD2 promoter and adapter containing a start codon, His-tag, and restriction sites for insertion of the gvpC gene fragments

This study

pARK-C1

pARK derivative with gvpC gene C1 fragment

This study

pARK-C2

pARK derivative with gvpC gene C2 fragment

This study

pARK-C3

pARK derivative with gvpC gene C3 fragment

This study

pARK-C4

pARK derivative with gvpC complete gene (C4)

This study

pDRK

Halobacterium sp. NRC-1 expression vector with gvpA promoter and adapter containing a start codon, His-tag, and restriction sites used for insertion of the gvpC gene fragments

This study

pDRK-C1-L

pDRK derivative containing gvpC gene C1 fragment fused to codon-optimized Gaussia princeps luciferase gene

This study

pDRK-C2-L

pDRK derivative containing gvpC gene C2 fragment fused to codon-optimized Gaussia princeps luciferase gene

This study

pDRK-C3-L

pDRK derivative containing gvpC gene C3 fragment fused to codon-optimized Gaussia princeps luciferase gene

This study

pDRK-C4-L

pDRK derivative containing gvpC complete gene (C4) fused to codon-optimized Gaussia princeps luciferase gene

This study