From: An improved genetic system for bioengineering buoyant gas vesicle nanoparticles from Haloarchaea
Strain/plasmid | Characteristics | Source or reference |
---|---|---|
Halobacterium sp. strain NRC-1 | Sequenced wild-type strain | Laboratory collection [28] |
Halobacterium sp. strain SD109 | Strain NRC-1 deleted for the gvp gene cluster of pNRC100 | Laboratory collection [13] |
Halobacterium sp. strain NRC-1Δura3 | Strain NRC-1 deleted for ura3 gene coding orotidine-5′-monophosphate | |
Halobacterium sp. strain NRC-1Δura3ΔgvpC | Strain NRC-1Δura3 deleted for the gvpC gene | This study |
pFM104gvpC::κ1 | Halobacterium-E. coli shuttle plasmid containing entire gvp gene cluster with κ insertion in the gvpC gene | Laboratory collection [11] |
pBB400 | Suicide plasmid capable of replication in E. coli but not Halobacterium, containing the ura3 gene | Laboratory collection [26] |
pBB400ΔgvpC | pBB400 plasmid containing gvpC gene-flanking regions for deletion construction | This study |
pMC2 | Halobacterium expression vector with cspD2 promoter and Halorubrum lacusprofundi β-galactosidase gene | Laboratory collection [27] |
pARK | Halobacterium sp. NRC-1 expression vector with cspD2 promoter and adapter containing a start codon, His-tag, and restriction sites for insertion of the gvpC gene fragments | This study |
pARK-C1 | pARK derivative with gvpC gene C1 fragment | This study |
pARK-C2 | pARK derivative with gvpC gene C2 fragment | This study |
pARK-C3 | pARK derivative with gvpC gene C3 fragment | This study |
pARK-C4 | pARK derivative with gvpC complete gene (C4) | This study |
pDRK | Halobacterium sp. NRC-1 expression vector with gvpA promoter and adapter containing a start codon, His-tag, and restriction sites used for insertion of the gvpC gene fragments | This study |
pDRK-C1-L | pDRK derivative containing gvpC gene C1 fragment fused to codon-optimized Gaussia princeps luciferase gene | This study |
pDRK-C2-L | pDRK derivative containing gvpC gene C2 fragment fused to codon-optimized Gaussia princeps luciferase gene | This study |
pDRK-C3-L | pDRK derivative containing gvpC gene C3 fragment fused to codon-optimized Gaussia princeps luciferase gene | This study |
pDRK-C4-L | pDRK derivative containing gvpC complete gene (C4) fused to codon-optimized Gaussia princeps luciferase gene | This study |