Figure 7
From: An improved genetic system for bioengineering buoyant gas vesicle nanoparticles from Haloarchaea
Partial map of pDRK-C-L plasmid series used for expression of gvp C-luciferase fusion genes in Halobacterium sp. NRC-1 and ∆ gvp C strains. The upper map (pARK-C series) displays the KpnI-BamHI region of the pARK-C series plasmids, with the cspD2 promoter labeled Pcs, His-tag shown as red box, and gvpC gene (with C1, C2, C3, and C4 regions marked) shown as blue arrow. The four lower maps (labeled pDRK-C1-L to C4-L) show the KpnI-BamHI regions of pDRK-C-L plasmid series containing the gvpA promoter (labeled Pgv), His-tag (red box), C1, C2, C3, and C4 regions of gvpC (blue boxes), and codon optimized Gaussia princeps luciferase gene (yellow arrow). The corresponding sites of KpnI, NdeI, and BamHI cleavage are indicated, while the AfeI sites at the GvpC-luciferase gene boundaries are not shown.