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Table 3 Strains and plasmids used in this study

From: Construction of fast xylose-fermenting yeast based on industrial ethanol-producing diploid Saccharomyces cerevisiaeby rational design and adaptive evolution

Strain/plasmid

Description

Referencea

CCTCC M94055

An industrial ethanol-producing S. cerevisiae strain; MATa

CCTCC

CIBTS0525

S. cerevisiae CCTCC M94055 derivative; arg1::pCpA1/G-XI; ty1::pYIE2-Ty-XI; δ::loxP-zeo-loxP-ADH1p-XKS1-XKS1t-TPI1p-TAL1-TAL1t-PGK1p-RPE1-RPE1t-FBA1p-TKL1-TKL1t-PDC1p-RKI1-RKI1t

This study

CIBTS0555

A single-colony isolate of CIBTS0525 after adaptive evolution

This study

CIBTS0573

CIBTS0525 derivative; gre3::pYIE2-GXF1

This study

CIBTS0735

A single-colony isolate of CIBTS0573 after adaptive evolution

This study

pCpA1/G-XI

Carrying TPI1p-XYLA-CYC1t, used to integrate a copy of Piromyces XYLA at the ARG1 locus

This study

pYIE2-Ty-XI

Carrying TPI1p-XYLA-CYC1t, used to integrate a copy of Piromyces XYLA at the Ty1 locus

This study

pSH47-hph

pSH47 encoding a hygromycin-resistant protein

This study

pYIE2-GXF1

Carrying TPI1p-GXF1-TPI1t, used to integrate Candida intermidia GXF1 at the GRE3 locus

This study

  1. aCCTCC, China Center for Type Culture Collection.