Construction of fast xylose-fermenting yeast based on industrial ethanol-producing diploid Saccharomyces cerevisiaeby rational design and adaptive evolution

Table 2 Performance of metabolically engineered S . cerevisiae strains in anaerobic batch cultivation

Strain	Description^a	Condition	Initial sugar, g/l	Final ethanol^b,c, g/l	Y_E^b,c, g/g	Y_G^b,c, g/g	R_S^b,c, g/l/h	R_E^b,c, g/l/h	Reference
RWB218	piXYLA; XKS1; TAL1; TKL1; RKI1; RPE1; ΔGRE3; adaptive evolution	Defined medium; inoculum size at 1.1 g DCW/l; fermentation time at 24 h	100 (glu.) 25 (xyl.)	47.5	0.38	0.08	5.21	1.98	[4]
H131-A3-AL^CS	piXYLA; psXYL3; psTAL1; TKL1; RPE1; RKI1; ARG4; LEU2; adaptive evolution	Defined medium; inoculum size at 0.05 g DCW/l; fermentation time at ca. 30 h	40 (xyl.)	16.4	0.41	N/A	1.33	0.55	[8]
GS1.11-26	cpxylA; XKS1; TAL1; TKL1; RPE1; RKI1; HXT7; TKL2; TAL2; psARAT, blaraB; ecaraA; ecaraD; adaptive evolution	Rich medium; inoculum size at1.3 g DCW/l; fermentation time at 13 h	36 (glu.) 37 (xyl.)	33.6	0.46	N/A	5.62	2.58	[18]
CIBTS0735	piXYLA; XKS1; TAL1; TKL1; RKI1; RPE1; ciGXF1; adaptive evolution	Rich medium; inoculum size at 0.63 g DCW/l; fermentation time at 16 h	40 (xyl.)	17.47	0.44	0.04	2.50	1.09	This study
		Rich medium; inoculum size at 0.63 g DCW/l; fermentation time at 24 h	80 (glu.) 40 (xyl.)	53.34	0.44	0.04	5.00	2.22	This study

^apiXYLA, Piromyces XYLA; psXYL3, Pichia stipitis XYL3; psTAL1, P. stipitis TAL1; cpxylA, Clostridium phytofermentans xylA; psARAT, P. stipitis ARAT; blaraB, Bacillus subtilis araB; ecaraA, Escherichia coli araA; ecaraD, E. coli araD; ciGXF1, Candida intermedia GXF1. ^bY_E, ethanol yield; Y_G, glycerol yield; R_S, sugar consumption rate; R_E, ethanol production rate. ^cValues for all strains were recalculated based on the whole fermentation process.

ISSN: 1472-6750