Figure 3

Schematic diagram of the selectable marker plasmid and co-transformed, non- selectable plasmids. (A) The chimeric selectable marker plasmid pPmr3. (B-D) Co-transformed, non-selectable plasmids containing reporter genes: (B) pPsaD-GLuc, (C) pHsp70A-GLuc, and (D) pHRLucP. The angled lines in (C) indicate introns. (A-D) Amplified genomic (gPCR) or RT-PCR fragments as well as probes used for Southern blots are indicated. V.c., Volvox carteri; C.r., Chlamydomonas reinhardtii; S.r., Streptomyces rimosus; G.p., Gaussia princeps; gluc, luciferase gene, pBS, pBluescript II vector.