Figure 2

Analysis of paromomycin resistance in wild-type and transgenic E. elegans strains. For analysis of antibiotic resistance, identical quantities of wild-type or transgenic Eudorina cells were exposed to increasing concentrations of paromomycin, and incubated for 10 days. Numbers refer to the concentration of paromomycin [μg/ml] utilized. Natural color (left) and red-shifted, false-color images (right) are shown. (A) Wild-type E. elegans strain UTEX 1193 used as a reference control. (B-D) Transgenic E. elegans strains co-transformed with pPmr3, the selectable marker plasmid, in addition to a second, non-selectable reporter gene plasmid. (B) Transformant EuTJ-14 was co-transformed with the plasmids pPmr3 and pPsaD-GLuc. (C) Transformant EuHR-7 was co-transformed with the plasmids pPmr3 and pHRLucP. (D) Transformant EuHsp-5 was co-transformed with the plasmids pPmr3 and pHsp70A-GLuc.