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Table 3 Activity and stability of the esterase in different organic solvents

From: Purification and characterization of an extracellular esterase with organic solvent tolerance from a halotolerant isolate, Salimicrobiumsp. LY19

Organic solvents

Log Powa

Residual activity (%)

Controlb

100 (3 d)c

Glycerol

−1.76

81.1 (2 d)

DMSO

−1.35

81.4 (1 d)

DMF

−1.0

68.9 (1 d)

Methanol

−0.76

18.4 (<1 h)

Acetonitrile

−0.34

11.9 (<1 h)

Ethanol

−0.3

15.5 (<1 h)

Acetone

−0.24

17.3 (1 d)

1-Butanol

0.88

67.9 (4 d)

Chloroform

1.97

62.8 (4 d)

Benzene

2.13

87.3 (4 d)

Toluene

2.73

64.1 (>5 d)

Cyclohexane

3.3

65.1 (>5 d)

n-Hexane

3.5

86.6 (>5 d)

1-Decanol

4.1

73.9 (>5 d)

Isooctane

4.7

119.1 (>5 d)

  1. aThe log Pow is the logarithm of the partition coefficient, P, of the solvent between n-octanol and water and is used as a quantitative measure of the solvent polarity.
  2. bThe activity of the purified esterase in the absence of organic solvents was taken as control (100%).
  3. cThe numbers in brackets are the half-lives of the enzyme in different organic solvents.