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Table 3 Activity and stability of the esterase in different organic solvents

From: Purification and characterization of an extracellular esterase with organic solvent tolerance from a halotolerant isolate, Salimicrobiumsp. LY19

Organic solvents Log Powa Residual activity (%)
Controlb 100 (3 d)c
Glycerol −1.76 81.1 (2 d)
DMSO −1.35 81.4 (1 d)
DMF −1.0 68.9 (1 d)
Methanol −0.76 18.4 (<1 h)
Acetonitrile −0.34 11.9 (<1 h)
Ethanol −0.3 15.5 (<1 h)
Acetone −0.24 17.3 (1 d)
1-Butanol 0.88 67.9 (4 d)
Chloroform 1.97 62.8 (4 d)
Benzene 2.13 87.3 (4 d)
Toluene 2.73 64.1 (>5 d)
Cyclohexane 3.3 65.1 (>5 d)
n-Hexane 3.5 86.6 (>5 d)
1-Decanol 4.1 73.9 (>5 d)
Isooctane 4.7 119.1 (>5 d)
  1. aThe log Pow is the logarithm of the partition coefficient, P, of the solvent between n-octanol and water and is used as a quantitative measure of the solvent polarity.
  2. bThe activity of the purified esterase in the absence of organic solvents was taken as control (100%).
  3. cThe numbers in brackets are the half-lives of the enzyme in different organic solvents.