|
Organic solvents
|
Log Powa
|
Residual activity (%)
|
|---|
|
Controlb
|
−
|
100 (3 d)c
|
|
Glycerol
|
−1.76
|
81.1 (2 d)
|
|
DMSO
|
−1.35
|
81.4 (1 d)
|
|
DMF
|
−1.0
|
68.9 (1 d)
|
|
Methanol
|
−0.76
|
18.4 (<1 h)
|
|
Acetonitrile
|
−0.34
|
11.9 (<1 h)
|
|
Ethanol
|
−0.3
|
15.5 (<1 h)
|
|
Acetone
|
−0.24
|
17.3 (1 d)
|
|
1-Butanol
|
0.88
|
67.9 (4 d)
|
|
Chloroform
|
1.97
|
62.8 (4 d)
|
|
Benzene
|
2.13
|
87.3 (4 d)
|
|
Toluene
|
2.73
|
64.1 (>5 d)
|
|
Cyclohexane
|
3.3
|
65.1 (>5 d)
|
|
n-Hexane
|
3.5
|
86.6 (>5 d)
|
|
1-Decanol
|
4.1
|
73.9 (>5 d)
|
|
Isooctane
|
4.7
|
119.1 (>5 d)
|
- aThe log Pow is the logarithm of the partition coefficient, P, of the solvent between n-octanol and water and is used as a quantitative measure of the solvent polarity.
- bThe activity of the purified esterase in the absence of organic solvents was taken as control (100%).
- cThe numbers in brackets are the half-lives of the enzyme in different organic solvents.
