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Table 2 Effects of metal ions and chemical reagents on esterase activity

From: Purification and characterization of an extracellular esterase with organic solvent tolerance from a halotolerant isolate, Salimicrobiumsp. LY19

Substances

Concentration (mM)

Residual activity (% ± SD)

Control

-

100

Ca2+

5

138.9 ± 1.6

Zn2+

5

97.6 ± 0.8

Fe2+

5

92.7 ± 1.7

Fe3+

5

91.8 ± 0.9

Cu2+

5

80.8 ± 1.8

Mn2+

5

97.5 ± 1.6

Hg2+

5

18.4 ± 0.4

Mg2+

5

97.7 ± 1.3

EDTA

1

8.9 ± 0.2

PMSF

1

11.1 ± 0.2

DEPC

1

92.1 ± 1.1

PAO

1

8.4 ± 0.3

β-mercaptoethanol

1

91.3 ± 1.5

  1. Residual activity was determined as described in “Methods” and expressed as the percentage of the control value (without any additives). Values are expressed as the averages of three independent experiments ± standard deviations.