|
Substances
|
Concentration (mM)
|
Residual activity (% ± SD)
|
|---|
|
Control
|
-
|
100
|
|
Ca2+
|
5
|
138.9 ± 1.6
|
|
Zn2+
|
5
|
97.6 ± 0.8
|
|
Fe2+
|
5
|
92.7 ± 1.7
|
|
Fe3+
|
5
|
91.8 ± 0.9
|
|
Cu2+
|
5
|
80.8 ± 1.8
|
|
Mn2+
|
5
|
97.5 ± 1.6
|
|
Hg2+
|
5
|
18.4 ± 0.4
|
|
Mg2+
|
5
|
97.7 ± 1.3
|
|
EDTA
|
1
|
8.9 ± 0.2
|
|
PMSF
|
1
|
11.1 ± 0.2
|
|
DEPC
|
1
|
92.1 ± 1.1
|
|
PAO
|
1
|
8.4 ± 0.3
|
|
β-mercaptoethanol
|
1
|
91.3 ± 1.5
|
- Residual activity was determined as described in “Methods” and expressed as the percentage of the control value (without any additives). Values are expressed as the averages of three independent experiments ± standard deviations.
