Purification steps | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
---|---|---|---|---|---|
Crude enzyme | 1020 | 57.1 | 17.9 | 1.0 | 100 |
60% (NH4)2SO4 | 725 | 17.3 | 41.9 | 2.3 | 71.1 |
DEAE-Cellulose | 361 | 4.4 | 82.0 | 4.6 | 35.4 |
Sephacryl S-100 | 149 | 1.2 | 124.2 | 6.9 | 14.6 |