Figure 9

Polymerization competency of HIV-1 CA. Purified CA (40-80 μM) was mixed with 50 mM sodium phosphate buffer (pH 8.0) and the polymerization was induced by the addition of 2.0 M NaCl solution. Polymerization was monitored by measuring absorbance at 350 nm every 10 s for up to 60 min (A). To further confirm that purified HIV-1 CA could be used for the identification of CA assembly inhibitors, we incubated 50 μM HIV-1 CA with varying concentrations (1,5,10 μM) of CA-specific anti-p24 MAb. Anti-Acetylcholinesterase antibody was used as a negative control (B).