Figure 6

Purification of HIV-1 CA expressed in NiCo21(DE3) E. coli . NiCo21(DE3)-pACYC-RIL expressing HIV-1 CA were grown in presence of 0.05 mM IPTG at 22°C for 12 hours and processed to purify recombinant protein using Co²⁺ resin, chitin resin, and Chitin + Co²⁺ resins. Sample collected during the procedure were analyzed by SDS-PAGE (A) and western blot (B). Lane M, BenchMark Pre-stained protein ladder; Lane M1, MagicMark XP Western Protein Standard; Lane 1, CA purified on Co²⁺ resin; Lane 2, CA purified on chitin resin; Lane 3, CA sequentially purified on chitin and Co²⁺ resins. Red arrowheads show contaminating proteins when CA was purified using Co²⁺ resin alone (Lane 1).