Figure 1

Construction and verification of pSA-Hp24-6His vector. We PCR amplified the p24 gene from pNL4.3 and cloned at NdeI/SacI restriction sites in inversely PCRed pMXB10 vector (A). Agarose gel electrophoresis analysis (B). Lane 1, 100 bp DNA ladder (NEB#N0467S); Lane 2, PCR amplified 714 bp p24; Lane 3, inversely PCRed 5.91 kb pSA vector; Lane 4, representative colony PCR (806 bp); Lane 5, restriction analysis of pSA-Hp24-6His with NdeI/SacI (5.89 kb vector backbone + 702 bp insert) ; Lane 6, 1 kb DNA ladder (NEB #N0468S).